Other
Part:BBa_K4286406:Design
Designed by: Bingru Feng Group: iGEM22_SZU-China (2022-09-02)
R.solani AG-3 ITS (ITS1 & 5.8SrRNA) sequence
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 322
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 322
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 322
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 322
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 322
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We take advantage of this conservative sequence of R.solani for the detection of rice sheath blight. Our LAMP-LFD system is able to reflect the existance of R.solani in infected rice.
Source
It comes from genomic sequence of Rhizoctonia solani AG-3.